Strategies for the Culture of CHO-S Cells: Simple- & Fed-batch Growth Utilizing a Hybrid-mode, Single-use Bioreactor

摘要

This technical note describes the application of two different strategies (simple- and fed-batch) for the culture of CHO-S cells using Cellexus Biosystems' (www.cellexusbiosystems. com) CellMaker PLUS bioreactor with disposable HybridBag. Simple-batchCulture: The first study involved the comparison of cultures grown in shaker flasks with that grown in the CellMaker PLUS using a HybridBag. A control culture was established in a 500 mL flask containing 50 mL CHO-S media (Gibco) at a cell density of 5x10~5 cells/mL. Concurrently, a culture was established in the CellMaker PLUS by inoculating 2.4 L CHO-S media in the HybridBag, also at 5x10~5 cells/mL.