Solution State Structure of pB1,the Mimotopic Peptide of Apolipoprotein B-100,by NMR

摘要

Apolipoprotein B-100(Apo-B100)is a major protein component for low density lipoproteins(LDL).A number of mimetic peptides of Apo-B 100 were screened from the phase-displayed random peptide library by utilizing monoclonal antibody(B9).Mimetic peptide for B9 epitope against apo B-100 was CRNVPPIFNDVYWIAF(pBl).From the BLAST search,the mimetic peptide pBl had 40% homology with apo B-100.As a result of the structural determination of this mimotope using homo/hetero nuclear 2D-NMR techniques and NMR-based distance geometry(DG)/molecular dynamic(MD)computations,DG structure had low penalty value of 0.3-0.6 A~2 and the total RMSD was 0.5-1.5 A.Moreover,pBl structure included a weak 3_(10)-helix from Ile~7 to Trp~(13).