A Sensitive Procedure for the Rapid Determination of Mandelic Acid by Flow Injection Analysis and Chemiluminescence Detection

摘要

A rapid and sensitive chemiluminescence (CL) method using flow-injection (FI) has been developed for the determination of mandelic acid which is based on the enhancement of mandelic acid to the CL intensity of Ru(bi-Py)_3~(2+)-Ce(IV) system. The enhancement effect was dependent on the concentration of mandelic acid, based on which, CL system was established for the determination of mandelic acid. The concentrations of Ru(bipy)_3~(2+) , Ce(IV), and H2SO4 were optimized. Under the optimum experimental conditions, the linear range and detection limit are 1.46-342.0 μg/mL and 0.072 μg/mL, respectively. The correlation coefficient (R) was 0.99732. The utility of this method was demonstrated by determining mandelic acid in capsules and human urine sample.