Gram-Scale Antibody Production:Using CHO Cell Transient Gene Expression via Flow Electroporation

摘要

In recent years researchers have turned to transient gene expression (TGE) as an alternative to CHO stable cell-line production for early-stage antibody development.There are variety of CHO cell transient transfection platforms, including engineeredCHO cells, unique expression constructs, specialized reagents, and proprietary technologies. These systems as well as highly optimized protocols for lipids and polyeth-ylenimine (PEI) have varying levels of reproducibility, scalability, and cost effectiveness, and in general produce antibody titers from 10 to 100 mg/L for a typical IgG.