Mouse Model Genetic Humanization:Model Generation Techniques and Humanized Mouse Applications

摘要

Genetic modification aimed at understanding the function andpathology associated with specific genes is most commonlyundertaken in mice, as about 99 percent of human genes have mouse or-thologs. Traditionally, the most popular transgenic technique has been the constitutive knock-out, wherein the gene of interest is disrupted in the mouse. However, constitutive inactivation can deem mice unviable, thereby precluding the study of the effects of the gene. Researchers developed the conditional knockout in response to this challenge, which allows a gene to be disrupted only in certain tissue or at a certain point in development.