Small open reading frames: Not so small anymore

摘要

Today, nearly 10 years after the publication of the complete sequence of the Saccharomyces cerevisiae genome, the total number of genes in this organism is largely considered resolved and currently stands at 5782 (http://www.yeastgenome.org/cache/ genomeSnapshot.html). This gene sequence information has led to construction of numerous yeast strain and plasmid collections, including the two-hybrid (Ito et al. 2000, 2001; Uetz et al. 2000) viable haploid deletion (Winzeler et al. 1999; Giaever et al. 2002), titratable promoter allele (Mnaimneb et al. 2004), and chromosomally tagged green fluorescent protein and TAP fusion libraries (Ghaemmaghami et al. 2003; Huh et al. 2003)