Dissecting Protein-Protein Interactions: SPR Facilitates the Understanding of Relationships That Can Lead to More Accurate Predictions

摘要

Nearly all cellular processes depend on protein-protein interactions. Therefore, understanding protein function and protein-protein interactions is key to gaining insights into the biochemical mechanisms that underlie disease and developing new drugs.Our current understanding of protein molecular recognition, however, remains far from comprehensive and impedes the drug development process. Label-free biosensor-based surface plas-mon resonance (SPR) systems are now commonly used in the study of diverse biomole-cular interactions, and have become, perhaps, the most widely accepted technology for quantifying their kinetic parameters, as these instruments allow real-time measurements. SPR analysis can be used, not only to provide fundamental insights into molecular recognition but throughout the protein therapeutic development process.