Revitalizing Surface Plasmon Resonance: Resurgence is Driven by SPR's Aptitude for Fragment-Based Lead Discovery

摘要

Surface plasmon resonance, or SPR, is used to measure changes in molecular weight. Thus, it has long been used to study protein-protein interactions by registering the association and dissociation of a ligand (prey) binding to an immobilized protein(bait). SPR has recently been revitalized by its aptitude for fragment-based lead discovery. Fragments are low-molecular-weight compounds (<350 kD) that bind to targets with low affinity (mu M-mM range), yet they exhibit high ligand efficiency—each atom contributes by directly contacting the target's binding site.