Possibility of Improved Salt Tolerance in Rice Transgenics Overexpressing pgNHXl

摘要

In the present study, transformants in rice were developed with PgNHXl gene following a tissue culture-independent in planta transf mation protocol. Analysis of TY plants by a stringent salt screening test at seedling and plant level identified putative transformai Integration of the transgenes in Ti generation plants was confirmed at the molecular level by PCR, genomic Southern and RT-Pi analysis. Physiological studies such as chlorophyll estimation and membrane permeability tests revealed that someof the Tj transfonna showed lower percent reduction in chlorophyll content and less membrane leakage compared to WT under salt stress. These results clea demonstrate that transgenic rice plants overexpressing PgNHXl, a vacuolar antiporter have better salt-tolerance. The stable integrat and inheritance of the transgene in subsequent T_2 generation was also confirmed by seed germination assay and PCR analysis.