Determination of Molecular Weight Distribution and Average Molecular Weights of Oligosaccharides by HPLC with a Common C18 Phase and a Mobile Phase with High Water Content

摘要

Size Exclusion Chromatography (SEC) has been extensively used to monitor molecular weight distribution of polymer samples. A typical phase of SEC is composed of uniform-sized porous beads with pores of wide dispersity. A solute whose size is larger than the largest pore of the phase will elute first, and a solute whose size is smaller than the smallest pore of the phase will elute last in SEC. In other words, the size factor governs elution order. Larger solutes will elute faster than smaller solutes.