Lipid Peroxidation Induced by the Reaction of Cytochrome c with Hydrogen Peroxide

摘要

Lipid peroxidation induced by the reaction of cytochrome c with H_2O_2 was investigated.When linoleic acid micelles or phosphatidyl choline liposomes were incubated with cytochrome c and H_2O_2,lipid peroxidation was increased in cytochrome c and H_2O_2 concentrations-dependent manner.Radical scavengers,azide,formate and ethanol prevented lipid peroxidation induced by the cytochrome C/H_2O_2 system.Iron specific chelator,desferoxamine also prevented the cytochrome C/H_2O_2 system-mediated lipid peroxidation.These results suggest that lipid peroxidation may be induced by the cytochrome C/H_2O_2 system via the generation of free radicals.Carnosine,homocarnosine and anserine are present in the muscle and brain of many animals and human.Previous studies show that these compounds have an antioxidant function.In the present study,carnosine,homocarnosine and anserine significantly prevented the cytochrome C/H_2O_2 system-mediated lipid peroxidation.Carnosine and related compounds also inhibited the free radical-generating activity of cytochrome c.The results suggest that carnosine,homocarnosine and anserine may prevent lipid peroxidation induced by the cytochrome C/H_2O_2 system through a free radical scavenging.