Enantiomer Separation of Acidic Chiral Compounds on a tert-Butylcarbamoylquinine-Silica Hybrid Monolith by Capillary Electrochromatography

摘要

A tert-butylcarbamoylquinine (tBuCQUI)-incorporated silica hybrid monolithic (QUI-SHM) capillary was prepared by "one-pot" approach via simultaneous sol-gel reaction of tetramethoxysilane and vinyltrimethoxysilane and free radical polymerization of tBuCQUI. A set of acidic chiral drugs were enantioseparated on QUI-SHM in mobile phases consisting of acetonitrile (ACN), methanol (MeOH), acetic acid (AcOH), and triethylamine (TEA). Influences of ACN/MeOH ratio, AcOH/TEA ratio, and their concentrations on enantioseparation were investigated. Baseline separations of enantiomers of all the compounds investigated were achieved with a mobile phase of 60:38 (v/v) ACN/MeOH containing 350 mM AcOH and 30 mM TEA under the applied voltage of -10 kV at 25 degrees C.