Hydrophobic Aminostyryl Quinolinium Dyes as New Fluorescent Stains for Proteins in Sodium Dodecyl Sulfate-polyacrylamide Gel

摘要

Fluorescent stains have been increasingly used for visualizing proteins in sodium dodecyl sulfate-polyacrylamide gels'(SDS-PAGE)because of their high sensitivity and ease of use.Among protein stains,dyes that bind non-covalently to protein-SDS complexes in SDS-PAGE are particularly useful for proteomic studies.Unlike covalent binders that modify proteins permanently,they maintain chemical composition of proteins.Therefore,post-gel microanalysis,such as protein characterization using mass spectrometry,is not hampered by their presence.