Proximity of Both Ends of Stems P3 and P4 of Self-kinasing RNA by ATP

摘要

The Kin.46 kinase ribozyme was selected for the transfer of the thiophosphate from ATPgammaS to its own 5' hydroxyl end.The reaction requires an oligonucleotide effector that is complementary to the 3' primer binding sequence (PBS) of the ribozyme and this effector served as reverse transcription primer during the amplification steps of the original selection for activity (Fig.I).