Selective Attachment of Mammalian Cells and Polystyrene Microbeads to Functional Amine Patterns Defined by Perfluoroalkyl Silane Surfaces

摘要

Surface modification technologies have been widely utilized for the investigation of the effect of surface properties on selective attachment of cells and cell functions.Recently,hydrophobic surfaces modified with organosilanes such as dichlorodimethyl silane and perfluoroalkyl silane have been shown to form a resistant barrier for cell attachment owing to their low surface energy.Moreover,they provide a chemically inert background and prevent the cross-contamination of liquid droplets on different spots.These characteristics have the potential to be utilized in the application of spot synthesis and array-type cell-based assays.To date,there have been a few reports on the formation of amine patterns defined by hydrophobic silane layers for the spatial organization of cells.However,several problems,such as the use of the limited types of organo-silane for the cell-adhesive patterns,complicated deep UV irradiation for the patterning,and possible contamination of patterns from organosilane coupling still remain to be overcome.Therefore,it is necessary to develop a new patterning technique that extends the versatile surface chemistry and eliminates the contamination problem.