Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7

摘要

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device.Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde.Through this covalent binding,up to 905 mug immunoglobulin G(IgG)per gram of glass beads was immobilized.For application,glass beads attaching antibody specific to Escherichia coli O157:H7,a foodborne pathogen,were packed into a microfluidic device and used for the detection of the serotype.This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.