PCR surveillance of paratuberculosis and a future strategy for the disease control with quantitative real-time PCR in Japan

摘要

The detection of Mycobacterium avium subsp. paratuberculosis (MAP) DNA from faecal samples by the quantitative real-time PCR (qPCR) seems to be crucially important as a rapid diagnosis of paratuberculosis, because infectious cattle continues to excrete a large amount of MAP into faeces, and causes environmental contamination with MAP during a time-consuming bacterial culture test. We have developed the sensitive and specific qPCR test for the diagnosis of paratuberculosis, and have performed an inter-laboratory validation of the qPCR test and the surveillance of bovine paratuberculosis by the qPCR.