Tsaokoarylone,a Cytotoxic Diarylheptanoid from Amomum tsao-ko Fruits

摘要

The crude methanol extract of the fruits of Amomum tsao-ko(Zingiberaceae)showed cytotoxic activity.Bioactivity-guided separation led to the isolation of a diarylheptanoid,tsaokoarylone [7-(4-hydroxy-3-methoxyphenyl)-l-(4-hydroxyphenyl)-hepta-4E,6E-dien-3-one](2).2 showed cytotoxicity at 4.9 and 11.4 mug/ mL(IC50)against human nonsmall cell lung cancer A549 and human melanoma SK-Mel-2,respectively,determined by SRB colorimetric method.During purification 6-(4-hydroxyphenyl)-4-hydroxyhexan-2-one(4)together with three known diaryIheptanoids was also isolated.Their structures were determined from interpretation of spectroscopic data(IR,UV,MS,and NMR)and synthesis confirmed the structure of 2.