Hybridization by an Electrical Force and Electrochemical Genome Detection Using an Indicator-free DNA on a Microelectrode-array DNA Chip

摘要

This research aims to develop DNA chip array without an indicator.We fabricated microelectrode array by photolithography technology.Several DNA probes were immobilized on an electrode.Then,indicator-free target DNA was hybridized by an electrical force and measured electrochemically.Cyclic-voltammograms(CVs)showed a difference between DNA probe and mismatched DNA in an anodic peak.Immobilization of probe DNA and hybridization of target DNA could be confirmed by fluorescent.This indicator-free DNA chip microarray resulted in the sequence-specific detection of the target DNA quantitatively ranging from 10~(-18)M to 10~(-5)M in the buffer solution.This indicator-free DNA chip resulted in a sequence-specific detection of the target DNA.