Ginsenoside Rbl Reduces Isoproterenol-Induced Cardiomyocytes Apoptosis In Vitro and In Vivo

摘要

Cardiomyocytes apoptosis can lead to heart failure. Conventional and alternative drugs, such as Chinese herbal remedies, have been developed to target cardiomyoblast cells apoptosis. In this study, we investigated the effects of ginsenoside Rbl (Rbl), an active compound, which is isolated from Notoginseng and Ginseng on isoproterenol-(ISO-) induced apoptosis in rat cardiomyocytes and its mechanism in vivo and in vitro. Rbl reduced the ISO-induced apoptosis in rat cardiomyocytes and H9c2 cells. The effect of Rbl was significantly suppressed by H89 (inhibitor for PKA), but not by C-l (inhibitor for PKC). Based on in-cell blot analysis, the ISO-induced PKA and PKC expressions were decreased by Rbl, which was inhibited by H89, but not by C-l. The expressions of caspase-3 and caspase-9 were decreased after treatment with both ISO and Rbl, but with no change for caspase-8. Our results indicated that Rbl reducing ISO-induced rat cardiomyocytes apoptosis may be involved in PKA and caspase-9 pathways.