Antidiabetic Effect of Oral Borapetol B Compound, Isolated from the Plant Tinospora crispa, by Stimulating Insulin Release

摘要

Aims. To evaluate the antidiabetic properties of borapetol B known as compound 1 (Cl) isolated from Tinospora crispa in normoglycemic control Wistar (W) and spontaneously type 2 diabetic Goto-Kakizaki (GK) rats. Methods. The effect of Cl on blood glucose and plasma insulin was assessed by an oral glucose tolerance test. The effect of Cl on insulin secretion was assessed by batch incubation and perifusion experiments using isolated pancreatic islets. Results. An acute oral administration of Cl improved blood glucose levels in treated versus placebo groups with areas under glucose curves 0-120 min being 72 ± 17 versus 344 ±10 mmol/L (P < 0.001) and 492 ± 63 versus 862 ± 55 mmol/L (P < 0.01) in W and GK rats, respectively. Plasma insulin levels were increased by 2-fold in treated W and GK rats versus placebo group at 30 min (P < 0.05). Cl dose-dependently increased insulin secretion from W and GK isolated islets at 3.3 mM and 16.7 mM glucose. The perifusions of isolated islets indicated that Cl did not cause leakage of insulin by damaging islet beta cells (P < 0.001). Conclusion. This study provides evidence that borapetol B (Cl) has antidiabetic properties mainly due to its stimulation of insulin release.