Detection of antibody response against Nocardia seriolae byenzyme-linked immunoabsorbent assay (ELISA) and a preliminary vaccinetrial in yellowtail Seriola quinqueradiata

摘要

Serum samples obtained from fish experimentally challenged or immunized with Nocardia seriolae were analyzed in order to evaluate the antibody response against N. seriolae. Indirect enzyme-linked immunoabsorbent assay (ELISA) was applied for antibody quantification with whole cell sonicated antigens (WCA) prepared from N. seriolae. The antibody responses were observed in yellowtail Seriola quinqueradiata and amberjack Seriola dumerili that were immersed in a suspension of N. seriolae cells. Although yellowtail immunized with formalin-killed cells (FKC) of N. seriolae or FKC with Freund's Incomplete Adjuvant (FKC-FIA) showed humoral responses against WCA, no protective effects resulting from the immunization with FKC or FKC-FIA were observed.