Solution conformation and dynamics of the O-antigen of the major lipopolysaccharide from Sinorhizobium fredii SMH12

摘要

The conformational and dynamic behavior of the O-antigen of the lipopolysaccharide of Sinorhizobium fredii SMH12, a wide-range host bacterium isolated from nodulated soybean plants growing in Vietnam, was studied. The O-antigen has a repeating unit consisting of the trisaccharide -> 4)-alpha-D-GalpA-(1 -> 3)-2-O-Ac-alpha -L-Rhap-(1 -> 3)-2-O-Ac-alpha-D-Manp-(1 ->, where the 0-6 position of the mannose residue in the repeating unit is unsubstituted, acetylated, or methylated in an approximate ratio 1:1:2. For the analysis of the conformational and dynamic behavior, the deacetylated polysaccharide was employed. A combination of NMR spectroscopic methods assisted by molecular mechanics and dynamic simulations was employed to deduce its major conformational and dynamic features. ((c) Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008).