Confirmation of IPNV from cell culture supernatants using a co-agglutination test.

Garden A.; Thompson F.; Smail DA

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Confirmation of IPNV from cell culture supernatants using a co-agglutination test.

机译：使用共凝集试验从细胞培养上清液中确认IPNV。

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A staphylococcal coagglutination (COA) test was compared to an enzyme linked immunosorbant assay (ELISA) for confirming the presence of infectious pancreatic necrosis virus (IPNV) in cell culture supernatant. There was close correlation between the COA test and ELISA at titres above 2.5x105 TCID50/ml. The COA test required little equipment, was quick, simple and reliable in use.

机译：将葡萄球菌凝集（COA）测试与酶联免疫吸附测定（ELISA）进行比较，以确认细胞培养上清液中是否存在传染性胰腺坏死病毒（IPNV）。滴度高于2.5x105 TCID50 / ml时，COA测试与ELISA密切相关。 COA测试几乎不需要设备，使用起来快速，简单且可靠。

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《Bulletin of the European Association of Fish Pathologists》 |2008年第3期|共4页
作者
Garden A.; Thompson F.; Smail DA;
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正文语种 eng
中图分类水产、渔业;
关键词
cell culture; coagglutination tests; ELISA; infectious diseases; necrosis; viral diseases; Infectious pancreatic necrosis virus; Staphylococcus; viruses;

机译：细胞培养;凝集试验;ELISA;传染病;坏死;病毒病;传染性胰腺坏死病毒;葡萄球菌;病毒;

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1. Confirmation of IPNV from cell culture supernatants using a co-agglutination test. [J] . Garden A., Thompson F., Smail DA Bulletin of the European Association of Fish Pathologists . 2008,第3期

机译：使用共凝集试验从细胞培养上清液中确认IPNV。
2. Enhanced method of herpes simplex virus culture confirmation using the virogen herpes slide test. [J] . L A Prymas, R A Venezia, M M McSharry Journal of Clinical Microbiology . 1987,第10期

机译：使用病毒原疱疹玻片试验确认单纯疱疹病毒培养的增强方法。
3. T-cell costimulatory capacity of oral and skin epithelial cells in vitro: presence of suppressive activity in supernatants from skin epithelial cell cultures. [J] . Hasseus B, Jontell M, Bergenholtz G, European journal of oral sciences . 2004,第1期

机译：口腔和皮肤上皮细胞在体外的T细胞共刺激能力：皮肤上皮细胞培养上清液中存在抑制活性。
4. Examination of Exosome purification method derived from culture supernatant of the mesenchymal cells from human ilium bone(hBMCs) which we chose, and its bone differentiation inducibility [C] . Yoshika Sugimoto, Yasuharu Yamazaki, Kazuno Moriyama, Society for Biomaterials annual meeting and exposition . 2018

机译：我们选择的人i骨（hBMCs）间充质细胞培养上清液中的外泌体纯化方法及其骨诱导诱导能力的研究
5. Degradation of human alpha- and beta-defensins by culture supernatants of Porphyromonas gingivalis. [D] . Carlisle, Matthew David. 2010

机译：牙龈卟啉单胞菌的培养上清液降解人α-防御素和β-防御素。
6. Enhanced method of herpes simplex virus culture confirmation using the virogen herpes slide test. [O] . L A Prymas, R A Venezia, M M McSharry 1987

机译：使用病毒原疱疹玻片试验确认单纯疱疹病毒培养的增强方法。
7. Table 1: Inhibitory spectrum of cell-free culture supernatant (CFCS) from LAB against Salmonella indicators by agar well diffusion test. [O] . -1

机译：表1：通过琼脂孔扩散试验从Lab对沙门氏菌指示剂的无细胞培养上清液（CFC）的抑制频谱。

Confirmation of IPNV from cell culture supernatants using a co-agglutination test.

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