High concentrations of mast cell chymase facilitate the transduction of the transforming growth factor-beta 1/Smads signaling pathway in skin fibroblasts

摘要

The aim of the present study was to investigate the effect of different concentrations of mast cell chymase on the transforming growth factor (TGF)-beta 1/Smad signaling pathway in skin fibroblasts. Cultured skin fibroblasts were treated with various concentrations of chymase for different time periods. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to assess the rate of cell proliferation. In addition, protein expression in the fibroblasts was measured using western blot analysis. Chymase was shown to enhance the proliferation of skin fibroblasts following incubation for 48, 72 and 96 h (P<0.01). Furthermore, high concentrations of mast cell chymase were shown to enhance the mRNA and protein expression levels of TGF-beta 1 after long-term (>= 6 h) incubation. In addition, high concentrations of mast cell chymase increased P-Smad2/3 and Smad2/3 protein expression. By contrast, low concentrations of mast cell chymase increased Smad7 protein. expression. Therefore, the results demonstrated that high concentrations of mast cell chymase facilitated the transduction of the TGF-beta 1/Smad signaling pathway in skin fibroblasts.