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首页> 外文期刊>Bulletin of the Veterinary Institute in Pulawy >Characterisation of Staphylococcus aureus and Staphylococcus aureus- like strains isolated from table eggs
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Characterisation of Staphylococcus aureus and Staphylococcus aureus- like strains isolated from table eggs

机译:从食用鸡蛋分离的金黄色葡萄球菌和金黄色葡萄球菌样菌株的鉴定

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摘要

The aim of the study was to provide a detailed phenotypic and genotypic characterisation of Staphylococcus aureus strains and the group of microorganisms with unusual biochemical patterns (called Staphylococcus aureus-Mks) isolated from table chickeneggs. All of the strains tested exhibited resistance to at least one of the 17 antibiotics tested, and 55.55% ofisolates were found to be resistant to five or more of them. PCR used for detection of the methiciilin resistance gene (mecA) confirmed the presence of a specific product of 533 bp in the case of two of the isolated S. auretis-liks strains. Analysis of the phylogenetic relationship between eight of 5. aureus and ten S. aureus-Wke strains distinguished 18 macrorestriction profiles following digestion with Smal endonuclease, indicating that there were no identical strains with the same macrorestriction profile. However, the presence of methicillin-resistant strains indicates a serious risk to consumer health.
机译:该研究的目的是提供详细的表型和基因型表征金黄色葡萄球菌菌株以及从餐桌鸡蛋中分离出的具有异常生化模式的微生物群(称为金黄色葡萄球菌-Mks)。所有测试的菌株均表现出对17种抗生素中至少一种的抗性,并且发现55.55%的分离物对其中5种或5种以上具有抗性。用于检测甲氧苄青霉素抗性基因(mecA)的PCR证实,在分离出的两个金黄色葡萄球菌样菌株中,存在533 bp的特异性产物。用Smal核酸内切酶消化后,对5个金黄色葡萄球菌中的8个和10个金黄色葡萄球菌-Wke菌株之间的系统发生关系进行了分析,从而区分出18个大限制性酶切图谱,这表明没有相同的菌株具有相同的大限制性酶切图谱。但是,耐甲氧西林菌株的存在表明消费者健康受到严重威胁。

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