DETERMINATION OF T-2 AND HT-2 TOXINS IN FEEDSTUFFS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH FLUORESCENCE DETECTOR

摘要

The procedure for the determination of T-2 and HT-2 toxins in feedstuffs has been developed. The test portion was extracted with methanol-water (80:20, v/v) after adding sodium chloride and cleaned-up through immunoaffinity column Mycotoxins were quantified by a high-performance liquid chromatography coupled with fluorescence detector (A,ex=381 nm, Xem=47C nm) after pre-column derivatisation, which was achieved with 4-dimethylaminopyridine and 1-anthroyi cyanide solutions. The method was validated according to the Commission Regulation (EC) No 401/2006. Average recoveries for the compounds rangec from 94.0% to 106.5% for T-2 toxin and from 80.3% to 102.9% for HT-2 toxin. The limits of detection and quantification for T-2 and HT-2 toxins were 6 and 15 fig kg"1, respectively.