CHARACTERISTICS OF GENES ENCODING STRUCTURAL PROTEIN OF POLISH STRAINS OF GOOSE PARVOVIRUS

摘要

The aim of this study was to come up with molecular characteristics of Polish strains of goose parvovirus (GPV) on the basis of genes encoding their structural proteins. Ten field GPV strains and two vaccine strains: B-38 and MFP were used. The PCR-RFLP method based on three distinct endonucleases: Hinjl, Mbol, and Rsal, which had restriction sites within the sequences of examined genes, was applied. It was found that the homology of VPl, VP2, and VPS among the studied strains ranged from 50% to 100%. The major differences in restriction patterns were found after application of HinfI, whereas 100% homology was observed after Rsal digestion. Significant differences were observed in restriction profiles of vaccine GPV strains. The study revealed thatthe application of PCR-RFLP for the analysis of VP1, VP2, and VP3 genes allowed for their molecular characteristics and differentiation between the vaccine and field strains.