Determination of alpha-ketoglutaric and pyruvic acids in urine as potential biomarkers for diabetic II and liver cancer

摘要

Background: A simple and sensitive hollow fiber-liquid phase microextraction with in situ derivatization method was developed for the determination of -ketoglutaric (-KG) and pyruvic acids (PA) in small-volume urine samples. 2,4,6-trichloro phenyl hydrazine was used as derivatization agent. Results: Under the optimum extraction conditions, enrichment factors of 742 and 400 for -KG and PA, respectively, were achieved. Calibration curves were linear over the range 1 to 1000 ng/ml (r(2) 0.998). Detection and quantitation limits were 0.03 and 0.02, and 0.10 and 0.05 ng/ml for -KG and PA, respectively. Conclusion: The concentrations in diabetic II and liver cancer samples were significantly lower than those from healthy people, showing their potential as biomarkers for these diseases.