Chlorpromazine transformation by exposure to ultraviolet laser beams in droplet and bulk

摘要

Multiple drug resistance requires a flexible approach to find medicines able to overcome it. One method could be the exposure of existing medicines to ultraviolet laser beams to generate photoproducts that are efficient against bacteria and/or malignant tumors. This can be done in droplets or bulk volumes. In the present work are reported results about the interaction of 266 nm and 355 nm pulsed laser radiation with microdroplets and bulk containing solutions of 10 mg/ml Chlorpromazine Hydrochloride (CPZ) in ultrapure water. The irradiation effects on CPZ solution at larger time intervals (more than 30 min) are similar in terms of generated photoproducts if the two ultraviolet wavelengths are utilized. The understanding of the CPZ parent compound transformation may be better evidenced, as shown in this paper, if studies at shorter than 30 minute exposure times are made coupled with properly chosen volumes to irradiate. We show that at exposure to a 355 nm laser beam faster molecular modifications of CPZ in ultrapure water solution are produced than at irradiation with 266 nm, for both microdroplet and bulk volume samples. These effects are evidenced by thin layer chromatography technique and laser induced fluorescence measurements. (C) 2015 Elsevier B.V. All rights reserved.