Semen analysis, especially estimation of sperm motility and morphology, is an important part of infertility investigation. The "Biologie Prospective" Nancy-France website (www.spermionet.com) proposes modern tools for clinical pathologists designed to promote common clinical practice and teaching by Internet. The aim of this study is describe these new techniques of on-line visualisation and diffusion for the study of human semen preparations. The virtual slide allows external quality control of sperm cytology. Video data of sperm motility are acquired by phase-contrast and dark field microscopy. The sequences are then compressed and published on line. The data have been evaluated by more than 480 laboratories. These new tools allow multicentre studies and collaboration based on standardization of semen analysis.
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