RFLP analysis as a tool for identification of lignicolous basidiomycetes: European polypores.

摘要

Restriction fragment length polymorphisms were generated for 52 species of lignicolous European polypores belonging to 31 different genera. An enzymatically amplified portion of the nuclear-encoded ribosomal DNA was found to be about 1800-1900 bp long for most taxa. After digestion with Hpa II, 44 distinct phenotypes were detected for the species under study; application of the additional restriction endonucleases, Hin6 I and Hinf I, resulted in 48 species-specific and two genera-specific phenotypes. The procedure described should allow for a reliable identification of unknown mycelia of lignicolous fungi.