Rugged and accurate quantitation of colchicine in human plasma to support colchicine poisoning monitoring by using turbulent-flow LC-MS/MS analysis

摘要

Background: Colchicine is a common drug used in inflammatory diseases. The narrow therapeutic index requires fast and reliable techniques for its quantitation. An online, automated sample preparation using TurboFlow? technology combined with triple-stage quadrupole MS detection was applied to identify colchicine in human plasma and follow intoxications. Methodology: Plasma samples (200 μl) were mixed with deuterated colchicine and protein precipitation ZnSO4 solutions. After centrifugation, supernatants were extracted onto a Cyclone P TurboFlow column and eluted onto a narrowbore Hypersil? GOLD column with a methanol/water gradient. Analytes were monitored in SRM mode (positive electrospray). Results: Total run time was 9.5 min. Calibration curves ranged from 0.342 to 17.1 ng/ml, with significant linearity (R2 0.99). Inter- and intra-assay precisions were 16.8% and accuracy was 84.4-110%. Conclusion: This method is suitable for monitoring intoxication in patients undergoing chronic treatment and is routinely applied to toxicological samples.