Preparation of a subcellular conjugate with the lipopolysaccharide from Vibrio cholerae 01 using beta-D-glucan as matrix.

摘要

A conjugate consisting of detoxified lipopolysaccharide of Vibrio cholerae, a carrier polysaccharide matrix and an immunogenic protein has been synthesised and the reaction conditions have been optimised for obtaining a high degree of conjugation. The obtained construct showed reactivity with the antibodies against V. cholerae and can serve as a prospective candidate for preparation of subcellular anti-cholera vaccine.