A chromogenic assay for coagulation factor VII: analytical performance characteristics and application in several diseases

摘要

A chromogenic assay for coagulation factor VII has been adapted for use on a Cobas Bio centrifugal analyzer. The interassay coefficients of variation ranged from 4.1 to 14. There was no effect on the factor VII amidolytic activity after freezing and thawing of the samples. The test was linear up to 200 factor VII. The correlation with a chronometric method for prothrombin time was acceptable (r=0.97,y=1.05x+5.5). The reference ranges for males (54–138) and females (56–200) were significantly different. The factor VII amidolytic activities were significantly elevated in males with non-insulin-dependent diabetes mellitus and in male hypertensives. No statistically significant increase was observed for female patients rather a decrease in females with insulin-dependent diabetes mellitus. Only in the male hypertensive patients and in the male patients with non-insulin-dependent diabetes mellitus were noticeable proportions of increased values seen (39.4 and 12.0). Nevertheless, the availability of a precise, rapid and easy to perform test for the determination of factor VII might be useful in the study of the role of this factor in the development of cardiovascular dise