Fed-batch production of carotenoids by Sporidiobolus salmonicolor (CBS 2636): kinetic and stoichiometric parameters

摘要

This work studied the fed-batch production of carotenoids by Sporidiobolus salmonicolor (CBS 2636) using agro-industrial waste as substrates (glycerol, corn steep liquor, parboiled rice waste). The feed rate was varied after 24 h of bioproduction, using glycerol (85 % pure and crude), peptone and malt extract in the feed. The maximum concentration of total carotenoids found was 4,400 mu g/L in 96 h of process at the conditions: 25 A degrees C, initial pH 4.0, stirring rate 180 rpm, aeration rate of 1.5 vvm (air volume per volume of culture medium per minute), 80 g/L glycerol, 15 g/L peptone and 5 g/L malt extract, with a feed rate of 112.5 mL each 12 h. The use of crude glycerol yielded maximum carotenoids of 3,009 A mu g/L. The specific production of carotenoids (Y (p/x) ) was 859.1 mu g/g, and maximum specific growth rate (mu (max)) was 0.05 h(-1). The highest cell production in this study was 0.085 g/L h.