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首页> 外文期刊>European journal of heart failure: journal of the Working Group on Heart Failure of the European Society of Cardiology >Myocardial gene expression alterations in peripheral blood mononuclear cells of patients with idiopathic dilated cardiomyopathy.
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Myocardial gene expression alterations in peripheral blood mononuclear cells of patients with idiopathic dilated cardiomyopathy.

机译：特发性扩张型心肌病患者外周血单个核细胞中的心肌基因表达改变。

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AIMS: To assess cardiac gene expression in peripheral blood cells of patients with idiopathic dilated cardiomyopathy (IDCM) and its relationship to echocardiographic left ventricular (LV) function. METHODS AND RESULTS: A complete echocardiographic study and blood sampling were performed in 65 consecutive stable IDCM patients with LV ejection fraction (LVEF) 31.76 +/- 10.07% and chronic mild to moderate heart failure (NYHA functional class II to III) for > or =9 months. Blood samples from 19 healthy individuals were included for comparison. Transcript levels of myocardin, GATA4, alpha- and beta-myosin heavy chain (MHC), sarcoplasmic reticulum calcium ATPase 2 (SERCA2), and phospholamban were determined by quantitative real-time reverse transcription-polymerase chain reaction. Myocardin (24.88 +/- 4.93 vs. 3.98 +/- 1.12, P = 0.0048) and GATA4 (17.85 +/- 4.85 vs. 0.45 +/- 0.15, P = 0.0069 x 10(-5)) were upregulated in IDCM patients compared with controls, whereas SERCA2 (5.11 +/- 0.42 vs. 8.93 +/- 1.07, P = 0.001) was downregulated. In IDCM patients, myocardin (r = 0.279, P = 0.025), GATA4 (r = 0.314, P = 0.011), beta-MHC (r = 0.444, P=0.0002), and alpha-MHC (r = 0.272, P = 0.034) showed positive correlations, whereas SERCA2 (r = -0.264, P = 0.034) exhibited a negative correlation with LVEF. Patients with elevated LV filling pressures had lower myocardin (15.06 +/- 3.10 vs. 43.12 +/- 12.03, P = 0.048), GATA4 (8.96 +/- 2.17 vs. 34.38 +/- 12.60, P = 0.026), beta-MHC (10.59 +/- 4.05 vs. 16.43 +/- 4.91, P = 0.013), and alpha-MHC (0.27 +/- 0.08 vs. 0.79 +/- 0.20, P = 0.033) and higher SERCA2 (5.65 +/- 0.54 vs. 3.90 +/- 0.61, P = 0.037) levels. Patients with atrial fibrillation (AF) had higher SERCA2 levels compared with sinus rhythm patients (6.75 +/- 0.84 vs. 4.54 +/- 0.45, P = 0.017). CONCLUSION: Our data indicate that cardiac gene expression alterations in peripheral blood cells of IDCM patients may reflect alterations in LV function, whereas the presence of AF may be associated with increased SERCA2 levels in these patients.
机译：目的：评估特发性扩张型心肌病（IDCM）患者外周血细胞的心脏基因表达及其与超声心动图左心室（LV）功能的关系。方法和结果：对65例连续稳定的IDCM患者进行了完整的超声心动图研究和血液采样，这些患者的LV射血分数（LVEF）为31.76 +/- 10.07％，且慢性轻度至中度心力衰竭（NYHA功能II至III级）≥ = 9个月。包括来自19个健康个体的血样用于比较。通过定量实时逆转录-聚合酶链反应测定了心肌素，GATA4，α-和β-肌球蛋白重链（MHC），肌质网钙ATPase 2（SERCA2）和磷脂质的转录水平。 IDCM患者的心肌蛋白（24.88 +/- 4.93对3.98 +/- 1.12，P = 0.0048）和GATA4（17.85 +/- 4.85对0.45 +/- 0.15，P = 0.0069 x 10（-5））被上调与对照组相比，SERCA2（5.11 +/- 0.42对8.93 +/- 1.07，P = 0.001）被下调。在IDCM患者中，心肌素（r = 0.279，P = 0.025），GATA4（r = 0.314，P = 0.011），β-MHC（r = 0.444，P = 0.0002）和alpha-MHC（r = 0.272，P = 0.034）显示正相关，而SERCA2（r = -0.264，P = 0.034）与LVEF呈负相关。左室充盈压升高的患者的心肌蛋白较低（15.06 +/- 3.10 vs.43.12 +/- 12.03，P = 0.048），GATA4（8.96 +/- 2.17 vs.34.38 +/- 12.60，P = 0.026），β- MHC（10.59 +/- 4.05与16.43 +/- 4.91，P = 0.013）和alpha-MHC（0.27 +/- 0.08与0.79 +/- 0.20，P = 0.033）和更高的SERCA2（5.65 +/- 0.54比3.90 +/- 0.61，P = 0.037）水平。与窦性心律患者相比，房颤（AF）患者的SERCA2水平更高（6.75 +/- 0.84对4.54 +/- 0.45，P = 0.017）。结论：我们的数据表明IDCM患者外周血细胞的心脏基因表达改变可能反映了LV功能的改变，而AF的存在可能与这些患者的SERCA2水平升高有关。

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