HPLC-DAD-ESI/MS identification of anthocyanins in Dioscorea trifida L. yam tubers (purple sachapapa)

摘要

The coloured tubers of purple sachapapa (Dioscorea trifida L.), a common crop in the subtropical and tropical regions, were analysed to characterise their anthocyanin composition. High-performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-ESI/MS) allowed the detection of 12 anthocyanin pigments. The compounds identified were derived from peonidin (Pn), cyanidin (Cy), and pelargonidin (Pg) aglycones, most of them showing the same substitution pattern, anthocyanin 3-acylglucoside-5-glucoside, where the acyl residue consisted of a hydroxycinnamic acid (i.e., ferulic or p-coumaric acid). The main anthocyanin found was peonidin 3-O-p-coumaroylglucoside-5-O-glucoside.