Determination of carotenoids and liposoluble vitamins in sea urchin(Paracentrotus lividus) by high performance liquid chromatography

摘要

High-performance liquid chromatography was used to determine carotenoids (lutein, p-carotene, beta -isocryptoxanthin, and beta -echinenone) and liposoluble vitamins (retinol=vitamin A, and alpha -tocopherol=vitamin E) in fresh and canned gonads of the edible sea urchin Paracentrotus lividus. beta -Carotene, beta -isocryptoxanthin, and beta -echinenone are vitamin A precursors. The carotenoids were determined with UV-vis detection and the vitamins with fluorescence detection. The sensitivity and precision of the method were good. Retinol was not detected in either fresh or canned material. Mean concentrations in fresh material (mg per 100 g dry weight) were 3.44 for lutein, 0.79 for beta -carotene, 1.89 for beta -isocryptoxanthin, 11.35 for beta -echinenone, and 7.86 for alpha -tocopherol. Mean concentrations in canned material were 70-80% of concentrations in fresh material, except for beta -echinenone, the concentration of which was about 30% higher in canned material.