The Effect of pH on Bromelain Partition from Ananas comosus by PEG400G7Phosphate ATPS

摘要

In this work, the thermodynamic equilibrium and applying of PEG4000/Phosphate ATPS on the purification of bromelain extracted from pineapple was studied. A rigorous study of the equilibrium curves and tie-line length from PEG400Q/phosphate ATPS were done for the pH 6-11 at 25 deg C. Results showed that there was augment in the PEG and salt contents with the high pH value from PEG4000/Phosphate ATPS and two-phase formation needed only increasing the PEG content. Two tie-line length at pH 11 from PEG4000/Phosphate ATPS were optimal condition for bromelain purification, one on composition of 14% PEG and 13% salt and other at 12.6% PEG and 12.2% salt, while a 25-62 folds of enzyme was found. SDS-PAGE electrophoreses had one band only, which showed thatbromelain was purified. Optimum conditions of bromelain use were found at pH 7 and between 30-40 deg C.