【24h】

Cloning and characterization of 5 ' upstream promoter region of rat WAP gene

机译:大鼠WAP基因5'上游启动子区的克隆与鉴定。

获取原文
获取原文并翻译 | 示例

摘要

Regulatory regions of genes encoding milk proteins are frequently used to produce in the mammary gland of transgenic animals a variety of pharmaceutically and medically important human proteins. One such example is the whey acidic protein (WAP) promoter region, identified so far in the genome of mouse, rat, rabbit, camel, pig, brushtail possum and Tammar wallaby. The aim of the present study was cloning and characterization of the 5' upstream promoter region of rat WAP gene. Using Genome Walking procedure, we cloned the region extending from -849 to -3671 bp. We have shown that there are two conserved regions highly similar to hypersensitive sites present in mouse and rabbit upstream region of WAP gene with binding sites for STAT5 transcription factor, essential for expression of WAP gene in mammary glands during lactation. We characterized dispersed and tandem repeats in the upstream region of rat WAP gen localized not far away from the translation initiation site. (C) 2004 Elsevier B.V All rights reserved.
机译:编码乳蛋白的基因的调节区经常用于在转基因动物的乳腺中产生多种药学和医学上重要的人蛋白。这样的例子之一是乳清酸性蛋白(WAP)启动子区域,到目前为止,该区域已在小鼠,大鼠,兔,骆驼,猪,刷尾负鼠和淡马小袋鼠的基因组中鉴定。本研究的目的是克隆和鉴定大鼠WAP基因5'上游启动子区域。使用基因组步行程序,我们克隆了从-849到-3671 bp的区域。我们已经表明,有两个高度保守的区域,与WAP基因的小鼠和兔子上游区域中的超敏位点高度相似,具有STAT5转录因子的结合位点,这对于泌乳期间WAP基因在乳腺中的表达至关重要。我们表征了大鼠WAP基因上游区域中离翻译起始位点不远的分散和串联重复序列。 (C)2004 Elsevier B.V保留所有权利。

著录项

站内服务
  • 论文查重
  • 论文收录引证报告
  • 文档翻译
  • 文档转换
获取原文

联系方式:18141920177 (微信同号)

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号