【24h】

GhHb1: A nonsymbiotic hemoglobin gene of cotton responsive to infection by Verticillium dahliae

机译:GhHb1:棉花的非共生血红蛋白基因,对大黄萎病菌的感染有反应

获取原文
获取原文并翻译 | 示例

摘要

Verticillium wilt of cotton is a widespread and destructive disease that is caused by the fungus pathogen Verticillium dahliae. Although no cotton cultivar is immune to the disease, some genotypes exhibit superior wilt tolerance. To gain an insight into the molecular mechanisms responsible for wilt tolerance, we employed the method of suppression subtractive hybridization (SSH) to isolate genes whose expression is up-regulated after inoculation of the pathogen in a wilt-tolerant cotton cultivar (Gossypium hirsutum cv. BD18). Among the identified candidate ESTs, a cDNA representing a nonsymbiotic hemoglobin gene (designated GhHb1) was further characterized in this study. Northern blot hybridization demonstrated that GhHb1 shares similar characteristics to some other nonsymbiotic hemoglobin genes including the hypoxic stress-induced expression. Sub-cellular localization analysis indicated that GhI4b1 proteins were predominantly present in the nucleus with a minor amount appearing in the cytoplasm. Two novel features of GhHb1 were also identified, indicating that GhHb1 expression is activated in the cotton roots after inoculation with V dahliae and that exogenous hydrogen peroxide induces GhHb1 expression. These results suggest that the GhHb1 may play a role in the defense response of G. hirsutum against V dahliae invasion. (c) 2005 Elsevier B.V. All rights reserved.
机译:棉花的黄萎病是一种由真菌病原体大黄萎病菌引起的广泛的破坏性疾病。尽管没有棉花品种对这种疾病免疫,但某些基因型表现出优异的抗枯萎性。为了深入了解造成青枯病的分子机制,我们采用了抑制消减杂交(SSH)方法,将病原菌接种到耐旱的棉花品种(陆地棉(Gossypium hirsutum cv。 BD18)。在已鉴定的候选EST中,在本研究中进一步表征了代表非共生血红蛋白基因(称为GhHb1)的cDNA。 Northern印迹杂交表明,GhHb1与某些其他非共生血红蛋白基因具有相似的特征,包括低氧应激诱导的表达。亚细胞定位分析表明,GhI4b1蛋白主要存在于细胞核中,少量出现在细胞质中。还确定了GhHb1的两个新特征,表明在接种大丽花后,GhHb1表达在棉花根部被激活,外源过氧化氢诱导GhHb1表达。这些结果表明GhHb1可能在G.hirsutum对大丽花入侵的防御反应中起作用。 (c)2005 Elsevier B.V.保留所有权利。

著录项

站内服务
  • 论文查重
  • 论文收录引证报告
  • 文档翻译
  • 文档转换
获取原文

联系方式:18141920177 (微信同号)

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号