首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Enzyme-linked immunosorbent assay for detection of Shiga toxin-producing Escherichia coli infection by antibodies to Escherichia coli secreted protein B in children with hemolytic uremic syndrome.
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Enzyme-linked immunosorbent assay for detection of Shiga toxin-producing Escherichia coli infection by antibodies to Escherichia coli secreted protein B in children with hemolytic uremic syndrome.

机译:酶联免疫吸附法可检测溶血性尿毒症综合征患儿的大肠杆菌分泌的蛋白B抗体检测产生志贺毒素的大肠杆菌感染。

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摘要

In order to detect immunoglobulin (Ig)A and IgG antibodies to Escherichia coli-secreted protein B in sera of children infected with Shiga toxin-producing Escherichia coli, an enzyme-linked immunosorbent assay was developed. The assay was tested using acute sera from 40 children with diarrhea-associated hemolytic uremic syndrome compared with 238 sera obtained from pediatric controls. Two cut-off values were used for children <5 (n=27) or > or =5 (n=13) years of age. Among the younger patients, 24 of 27 had IgA antibodies to Escherichia coli-secreted protein B (sensitivity, 89%; specificity, 98%) and 22 of 27 had IgG antibodies (sensitivity, 82%; specificity, 94%). Among the older patients, 13 of 13 had IgA antibodies (sensitivity, 100%; specificity, 96%) and 11 of 13 had IgG antibodies (sensitivity, 85%; specificity, 96%). This enzyme-linked immunosorbent assay detects Shiga-toxin-producing Escherichia coli independent of serogroup and could serve as a complementary assay for detection of infection.
机译:为了检测感染产志贺毒素的大肠杆菌的儿童血清中针对大肠杆菌分泌的蛋白B的免疫球蛋白(Ig)A和IgG抗体,开发了一种酶联免疫吸附试验。使用来自40例腹泻相关溶血性尿毒症综合征患儿的急性血清与从儿科对照组获得的238份血清进行了比较,对本试验进行了测试。 <5(n = 27)或>或= 5(n = 13)岁的儿童使用了两个临界值。在较年轻的患者中,27名患者中有24名具有针对大肠杆菌分泌的蛋白B的IgA抗体(敏感性为89%;特异性为98%),而27名患者中有22名具有IgG抗体(敏感性为82%;特异性为94%)。在老年患者中,13名患者中有13名具有IgA抗体(敏感性为100%;特异性为96%),13名患者中有11名具有IgG抗体(敏感性为85%;特异性为96%)。这种酶联免疫吸附测定法可检测独立于血清群的产志贺毒素的大肠杆菌,并可作为检测感染的补充测定法。

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