Molecular Structure the Elelctron Spectroscopic Way

摘要

With neither X-ray crystallographic nor nuclear magnetic resonance data, it is still possible to generate important structural data on macromolecular complexes Electron spectroscopic imaging (ESI) is an analytical technique, based on electron microscopy, which can generate structural information. In essence, ESI detects low molecular weight elements—such as phosphorus—and correlates their positions with molecular structures seen under the electron microscope. It can address questions at the eel lular and even the molecular level of detail by providing images of extremely high contrast without conventional stains. ESI also gives high resolution maps of particular chemical elements in a biological specimen or macromolecular complex.