Cloning and expression in Escherichia coli of the gene encoding Streptomyces PMF PLD, a phospholipase D with high transphosphatidylation activity

摘要

Phospholipases D (PLDs) hydrolyze phospholipids to yield phosphatidic acid and the corresponding alcohol and catalyze a transesterification (transphosphatidylation) reaction when alcohol is present as a nucleophilic donor. Bacterial forms of PLDs have shown to be suitable as biocatalysts for the synthesis of phospholipid derivatives of industrial interest.