Fox grape cv. Bordo (Vitis labrusca L.) and grapevine cv. Chardonnay (Vitis vinifera L.) cultivated in vitro under different carbohydrates, amino acids and 6-benzylaminopurine levels.

摘要

The aim of this work was to study the influence of sucrose and glucose, amino acids and BAP (6-Benzylaminopurine) levels on in vitro shoot regeneration of fox grape cv. Bordo and grapevine cv. Chardonnay. The nodal segments from micropropagated material were used as explants and half-strength MS medium as the basal medium. Sucrose and glucose at 15, 30 and 45 g.L-1were tested as a carbon source and the supplementation of adenine, asparagine, alanine, glycine, cysteine, glutamine, arginine was tested at 40 g.L-1. The BAP levels (1 and 5 micro M) in solid and double-phase media were evaluated and compared with a control medium without BAP. Bordo had best in vitro growth than Chardonnay. Sucrose was a better carbohydrate source than glucose for both the cultivars. Bordo and Chardonnay had different amino acid preferences for some parameters. In conclusion, for in vitro shoot regeneration from the nodal segments, culture on solid medium with 5 micro M BAP, 15 g.L-1 sucrose for Bordo and 45 g.L-1sucrose for Chardonnay showed better results. Similarly, the supplementation of 40 g.L-1 arginine for Bordo and 40 g.L-1 arginine or glycine for Chardonnay showed better results.