Degradation and detoxification of acid orange 52 by Pseudomonas putida mt-2: a laboratory study

摘要

Introduction Acid orange 52 (A052), extensively used in textile industries, was decolorized by Pseudomonas putida mt-2. A052 azoreduction products such as N,N'-dimethyl-p-phenylenediamine (DMPD) and 4-aminobenzenesulfonic acid (4-ABS), were identified in the static degradation mixture. These amines were identified only in media of static incubation, which is consistent with their biotransfor?mation under shaken incubation (aerobic conditions). Materials and methods Tests with azo products were carried out, and whole cells were found able to easily degrade DMPD contrary to 4-ABS. However, this last could be attacked by cell extract, and an oxygen uptake was observed during the reaction.