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Combining An Artificial Break In Brood Rearing With Oxalic Acid Treatment To Reduce Varroa Mite Levels

机译:结合人工育雏和草酸处理降低螨虫螨虫水平

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Sixty-two colonies were set up to test the hypothesis that Varroa control with oxalic acid (OA) can be enhanced by combining treatment with late summer requeening. Each colony consisted of two full depth boxes with nine frames per box. All colonies were queen right at the beginning of experiment and had brood present. Approximately 300 adult bees were collected pre- and post-treatment to calculate mites-per-bee. This experiment consisted of four treatment groups, requeen plus OA, requeen only, OA only, and controls (untreated). Queens were caged in the requeen treatment groups five days prior to placing a sealed queen cell in the colonies. This provided a period of 18-21 days without egg lying, which allowed most of the brood present to emerge. A1.7oz (50 ml) application of a 3.0% OA sugar water solution (suganwater) (1:1) (w:w:w) was applied to the requeen plus OA, and OA only treatment groups. The OA solution was trickled from above the frames into the bee-ways with a 3.4 oz (100 ml) syringe. Post-treatment alcohol samples were taken four days after treatment. The results indicate that combining late summer requeening with OA treatment significantly reduced mites compared to untreated colonies, colonies that are requeened but not treated, and colonies only treated with OA. Although we had a significant decrease in the treatment group that was requeened and treated with OA, we found evidence that the presence of brood is not the only factor affecting the efficacy of OA treatments.
机译:建立了62个菌落,以检验这样的假说:将草酸(OA)与治疗结合使用,可以增强夏至更生。每个菌落由两个全深度的盒子组成,每个盒子有九个框架。在实验开始时,所有菌落都处于女王状态,并且存在育雏。治疗前后大约收集了300只成年蜜蜂,以计算每只蜜蜂的螨虫数量。该实验由四个治疗组组成,重新治疗加OA,仅重新治疗,仅OA和对照组(未治疗)。在将密封的皇后细胞放入菌落之前五天,将皇后区关在重蚁处理组中。这提供了18到21天的时间,没有产卵,这使得大部分育雏出现。将3.0%OA糖水溶液(suganwater)(1:1)(w:w:w)的A1.7oz(50 ml)溶液用于requeen加OA和仅OA的治疗组。用3.4盎司(100毫升)的注射器将OA解决方案从框架上方滴入蜜蜂通道。治疗后四天采集治疗后的酒精样品。结果表明,与未处理的菌落,需要重新处理但未处理的菌落以及仅用OA处理的菌落相比,夏末重制与OA处理的结合显着减少了螨虫。尽管我们在重新申请并用OA治疗的治疗组中有明显减少,但我们发现有证据表明,育雏并不是影响OA治疗功效的唯一因素。

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