Use of DNA barcoding for host plant identification

摘要

We present a molecular approach for identification of Dutch elm (Ulmus glabra) as host plant of some species of European elm beetles (Scolytus) using DNA sequences of the chloroplast rbcL gene and trnL intron. The samples analysed were obtained from a piece of infested bark collected from a bridge in France. Molecular identification was consistent with microscopical analysis and with reference material collected in The Netherlands. When sequences were included of all trees surrounding the bridge, the same results were obtained. DNA barcoding therefore seems a promising new tool for increasing knowledge of plant-insect interactions.