Use of Chitosan Gel for the Purification of Protein

摘要

The aim of this study was to optimize the preparation of chitosan gel and to use it for protein purification. Theoptimized preparation parameters were chitosan concentration 2.0%, glutaraldehyde concentration 0.6%, lowcross-linking rate, NaOH concentration 1.6%, amount ofNaBH_4 0.4 g. In order to use the chitosan gel, the elution .conditions were optimized as follows. NaCl concentration 0.05 mol/L in the tris-HCl (pH 9.05) at the flow rate of2.03.0 mL/min. Particle size of chitosan gel was 120-140 jj.m. Neutral protease could be separated into fouringredients through chitosan gel column. The yield of enzyme was more than 90%. Albumin bovine serum could beseparated into two ingredients through gel column and the total yield of albumin bovine serum was more than 70%.